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By transfecting p53 R175H into non-transformed mouse embry-onic ?broblasts (MEF) (Trp53?/?, Mdm2?/?) with CHIP or Mdm2 along withHsp70, these authors showed that Hsp70 promotes the degradation of mutant p53through CHIP but inhibits the degradation of mutant p53 by Mdm2. At daily intervals, the number of cells in control and Dex-treated samples was determinedby scrapping off the adherent cells (viable) and counting them under a light microscope.
Gradually, to my relief, the diet began to kick in,enabling me to decrease my dosage. Tuberculous spondylitis: risk factors and clinical/paraclinical aspects inthe south west of Iran. Textbook of gastrointestinal radiology.Philadelphia: Saunders; 2008.